Key words: Listeria monocytogenes, poultry, processing plant, evisceration. ABSTRACT . Listeria antisera (Denka Seiken, Tokyo, Japan), accord- ing to the . Caracterização feno e genotípica de cepas de Listeria monocytogenes isoladas They were also serotyped (Denka Seiken, Japan), and sub–typed by PFGE. Antisera for the typing of Listeria Group O and H antigens. Aids public health and food testing laboratories in identifying sources of infection/contamination to.
|Published (Last):||15 August 2005|
|PDF File Size:||8.71 Mb|
|ePub File Size:||3.90 Mb|
|Price:||Free* [*Free Regsitration Required]|
We determined that the O-factor and H-factor antisera gave suitable results at dilutions of 1: Additionally, there were increased values for sulfamethoxazole MICs Table 1. Most industrialized countries have developed surveillance systems for listeriosis since 25following outbreaks that demonstrated the importance of the foodborne transmission of lizteria disease. In this study, these two drugs oisteria tested individually and none of the strains showed resistance to trimethoprim.
Cells were then washed, pelleted by centrifugation, and resuspended in 0. Modifications to this scheme proposed by Garcia et al. In addition, mixed-serotype cultures of L.
Manufacturer of diagnostic reagents and vaccines,DENKA SEIKEN | Others
The antimicrobial susceptibility of the isolates was evaluated by the microdilution broth method lsteria ampicillin, gentamicin, trimethoprim, sulfamethoxazole and vancomycin. After digestion with ApaI and AscI, the strains were distributed in 3 different groups according to their profile. Combined ribotyping and random multiprimer DNA analysis to probe the population structure of Listeria monocytogenes.
In addition, formaldehyde-treated cells were prone to give false-positive reactions to O antiserum IX data not shown. We sought to adapt a commercial serotyping kit to a format that would be cost-effective, reliable, and of sufficiently high throughput to facilitate serotype determination of L.
Survival of pathogenic bacteria during mesophilic anaerobic digestion of animal waste.
During testing, two strains FSL-J and G were nonmotile on motility agar, and a third strain FSL-J was delayed in motility, requiring a second passage on motility ljsteria. Nenhuma das cepas foi resistente aos antimicrobianos testados; entretanto, sete tiveram valores da CIM aumentados para o sulfametoxazol.
The analysis of macrorestriction patterns obtained by PFGE lixteria the combination of the results obtained with both restriction enzymes, ApaI Fig. In Brazil, few clinical cases of listeriosis have been reported and none of them had their source of infection established.
Finally, it is known that the cases documented here may not represent the true incidence in the community, especially with regard to perinatal infection, once many lisetria of the region covered by this study do not often send the isolates for further characterization by the Regional Lab.
Serological analysis of the genus Listeria.
Traditional epidemiological surveillance alone may not detect many common source outbreaks, particularly if a limited number of cases occur over a wide geographic area, due to the unique characteristics of human foodborne listeriosis. Susceptibilidade aos antimicrobianos de Listeria spp.
Isolation and detection of Listeria monocytogenes using fluorogenic and chromogenic substrates for phosphatidylinositol-specific phospholipase C. Epidemiology of human listeriosis. Palumbo1 Monica K. Wild birds and silage as reservoirs of Listeria in the agricultural environment.
National Committee for Clinical Laboratory Standards: Prevalence and fingerprinting of Listeria monocytogenes strains isolated from raw whole milk in farm bulk tanks and in dairy plant receiving tanks. A rapid method for the identification and partial serotyping of Listeria monocytogenes listeeia food by PCR and restriction enzyme analysis. All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License. National Center for Biotechnology InformationU.
However, this current favorable situation of quasiuniform susceptibility to the antibiotics weiken in clinical practice may deteriorate under the selective pressure exerted by overuse of the drugs in the country.
DENKA SEIKEN Co.,Ltd. | Salmonella
Identification of mixed-serotype listerka. Note that serotype 4e is indistinguishable from 4b in this case.
Serotyping of Listeria monocytogenes and related species. Therefore, a more accurate study of such infection is necessary in our country. Initial comparisons of the serotyping methods using L. The thirteen isolates belonged to three serotypes: Influence of environmental conditions on the expression of virulence factors by Listeria monocytogenes and their use in species identification.
More-discriminatory molecular methods are currently in use 7101213162022 and offer a greater degree of confidence in determining epidemiological relatedness of food-borne and clinical L. This method differentiates L.
Virulence characteristics of Listeria monocytogenes. Listeriosis has been mainly reported by those countries, with few or even no reports from Africa, Asia and South America.
All subsequent steps were performed at room temperature. The use of a commercially prepared set of antisera and a semiquantitative ELISA format greatly reduce the variability of antiserum quality as well as the inconsistencies in judgement associated with weakly agglutinating antigen-antiserum combinations.
Subtyping methods will only provide their full public health benefit if food histories were routinely obtained for all listeriosis patients to provide an epidemiological support for putative single genotype clusters.
For example, single-colony picks from an enrichment culture from one soil sample showed that the soil contained two different serotypes of L. In addition to those strains serotyped by both methods, 97 L. Serotyping of 80 strains from the W.
Using a well enzyme-linked immunosorbent assay ELISA format instead of agglutination as a means to score reactions with each antiserum, this method provides a semiquantitative measurement of positive and negative reactions and requires only a fraction of the antisera used in the agglutination assay. With the exception of a strain previously serotyped as 4e by agglutination, which was consistently serotyped as 4b by ELISA, strains representative of all serotypes were typeable by this method.